| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Anti-Metastatic Properties of a Marine Bacterial Exopolysaccharide-Based Derivative Designed to Mimic Glycosaminoglycans
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| Autores: |
Heymann, Dominique
Ruiz-velasco, Carmen
Chesneau, Julie
Ratiskol, Jacqueline
Sinquin, Corinne
Colliec-jouault, Sylvia
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| Data: |
2016-03
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| Ano: |
2016
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| Palavras-chave: |
Exopolysaccharides
Glycosaminoglycan
Heparin-like
Derivatives
Sulfation
Bone metabolism
Bone remodeling
Lung mestatases
Osteosarcoma
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| Resumo: |
Osteosarcoma is the most frequent malignant primary bone tumor characterized by a high potency to form lung metastases. In this study, the effect of three oversulfated low molecular weight marine bacterial exopolysaccharides (OS-EPS) with different molecular weights (4, 8 and 15 kDa) were first evaluated in vitro on human and murine osteosarcoma cell lines. Different biological activities were studied: cell proliferation, cell adhesion and migration, matrix metalloproteinase expression. This in vitro study showed that only the OS-EPS 15 kDa derivative could inhibit the invasiveness of osteosarcoma cells with an inhibition rate close to 90%. Moreover, this derivative was potent to inhibit both migration and invasiveness of osteosarcoma cell lines; had no significant effect on their cell cycle; and increased slightly the expression of MMP-9, and more highly the expression of its physiological specific tissue inhibitor TIMP-1. Then, the in vivo experiments showed that the OS-EPS 15 kDa derivative had no effect on the primary osteosarcoma tumor induced by osteosarcoma cell lines but was very efficient to inhibit the establishment of lung metastases in vivo. These results can help to better understand the mechanisms of GAGs and GAG-like derivatives in the biology of the tumor cells and their interactions with the bone environment to develop new therapeutic strategies.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00333/44442/44112.pdf
https://archimer.ifremer.fr/doc/00333/44442/44113.pdf
DOI:10.3390/molecules21030309
https://archimer.ifremer.fr/doc/00333/44442/
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| Editor: |
Mdpi Ag
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| Formato: |
application/pdf
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| Fonte: |
Molecules (1420-3049) (Mdpi Ag), 2016-03 , Vol. 21 , N. 3 , P. -
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| Direitos: |
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
info:eu-repo/semantics/openAccess
restricted use
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